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The excessive ROS production during reperfusion can lead to the opening of the mitochondrial permeability transition pore (mPTP), causing mitochondrial swelling, loss of membrane potential, and cell death [2]
Remove the cap from the bacteriostatic water vial and wipe the rubber stopper with an alcohol prep pad
Wilder was the first to designate this unique dietary approach as KD [13]
The pellet was further lysed in buffer B (3 mM EDTA, 0.2 mM EGTA, 1 mM DTT and protease inhibitor cocktail) for 30 min on ice, followed by centrifugation at 1,700 g for 5 min
Methods: Tamoxifen-resistant cell lines previously developed and characterized in our laboratory were metabolically profiled with nuclear magnetic resonance spectroscopy (NMR) using cryogenic probe, and the findings were correlated with the expression of genes that encode the key enzymes of the significant metabolites
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