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Cells were fixed with 4% PFA for 30 minutes, and intracellular glycogen was stained according to the manufacturers instructions

These in-organ changes in energy metabolism are also reflected in the plasma metabolome after ischemic AKI, which is characterized by the depletion of numerous amino acids and energy substrates 6

This results in sustained release, better tissue targeting, stronger mitochondrial protection, and improved redox balance.

Dervaux and F