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Cultures were pulse-labeled with 10 M BrdU (a nucleoside analog that competes with thymidine for incorporation into DNA) in DMEM-10% HS for the final 2-h period of 48-h in culture, followed by immunocytochemistry for detection of BrdU using G3G4 anti-BrdU mAb (1:100 dilution) and HRP-conjugated anti-mouse IgG Ab (1:500 dilution)

For chronic conditions, some people use longer cycles

Refrigerate upon opening

The test, which very recently received CE certification and is available in several European and middle eastern countries, is the first non-invasive approved test for endometriosis

7A), synapses with narrow synaptic clefts and sharply defined, electron-dense postsynaptic densities (PSDs), and presynaptic terminals containing abundant synaptic vesicles (SVs) clustered near active zones (Fig