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Data were acquired using an MS3-based TMT method using the following scan parameters: scan sequence began with an MS1 master scan (Orbitrap analysis, resolution 120,000, 4001700 m/z, RF lens 60%, automatic gain control [AGC] target 2E5, maximum injection time 50 ms, centroid mode) with dynamic exclusion enabled (repeat count 1, duration 15 s)
Particles for holo-complex or sub-complex were selected separately and re-extracted into the original pixel size of 1.08
DBI is processed into smaller fragments, and one of these octadecaneuropeptide (ODN) is thought to drive its appetite-suppressing effects
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