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The coordinates of NTSR, G i , and scFv16 from the structure of the neurotensin-NTSR-G i -scFv16 complex (PDB ID 6OS9) were used as initial models to dock into the cryo-EM maps using Chimera 48

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Redox status analysis The concentration of CYS, cystine (CYSS), GSH, and GSSG in the red blood cell, the liver, the mucosa of the intestine at 5 and 75% of small intestinal length, as well as their concentrations in the digesta of the stomach, SI1, SI2, and SI3 were measured according to the method from Jones et al