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Inyang KE, Szabo-Pardi T, Wentworth E et al (2019) The antidiabetic drug metformin prevents and reverses neuropathic pain and spinal cord microglial activation in male but not female mice
Preparation Condition 3 (EDTA 80 o C deproteinization): Deproteinization may not be necessary to prevent spontaneous oxidation of free GSH Unexpectedly, we found no difference in the levels of total GSH, free GSH, GSSG, or the ratio of free GSH to GSSG between the EDTA-treated plasma samples which were stored at 80 o C (Preparation Condition 2 and Preparation Condition 3)
Getting the documentation right is the second and equally important step
For detailed information on what BPC-157 is and how it was discovered, that guide provides thorough background