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in order to limit time to freezing (and potential changes in metabolic phenotype due to death) no additional processing of tissue occurred and organs were not perfused prior to collection

When B12 is low, red blood cells become oversized and inefficient (megaloblastic anemia), and oxygen delivery to follicles drops

The infusion process typically takes around 45-60 minutes, during which you can relax, read, or even work

This is known as hypercobalaminemia

FIGURE 1 On the surface, the mice after GPX1 KO performed normally, but these mice were susceptible to the production of oxidative substances, and when stimulated, they were easy to cause harm to mice and increase oxidative stress in vivo