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The next day, samples were incubated with Alexa Fluor 488-conjugated anti-IgG secondary antibodies and Alexa Fluor 647-conjugated anti-IgG for 1 h and stained with DAPI for 7 min

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Public health impact According to the Centers for Disease Control and Prevention (CDC), in 2023, 40.1 million people (one in eight) had diabetes

Leaky gut allows partially digested food proteins to enter systemic circulation, triggering IgG-mediated delayed immune reactions that produce inflammatory responses to previously tolerated foods