Cryo-EM sample preparation and data acquisition Four microliters of the sample (protein concentration: 3 mg/mL, liposome concentration: 1.5 mg/mL) were loaded onto a Quantifoil Au grid (R2/2, 300 mesh), followed by plunge-freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher) at 4 C and 100% humidity
Different forms require different handling
Use a chemical exfoliant 23 times this week
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Wyce A, Bai Y, Nagpal S, Thompson CC
